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The plant material was collected on 34 individuals growing in the Dendrological Garden of Poznań University of Life Sciences (52°25'32,95" N 16°53'39,83" E) and Botanical Garden of Adam Mickiewicz University in Poznań (52°25'11,70" N 16°52'55,07" E). The species for this study originated from Europe, Asia Minor, central and eastern Asia and North America and included: Abies alba,...
Adaptation to local environmental gradients is one of main characteristics of living organisms. Scots pine (Pinus sylvestris L.) is the most widely distributed conifer in the world and main forest forming component in Europe. Based on genetic, morphological and growth performance traits, several local ecotypes of the species were distinguished across the species distribution range. The existence of...
The study showed the genetic structure of nine Schrenk spruce stands, which represented altitude and geographical variants in the Tien−Shan mountains in Kyrgyzstan. Comparison between genetic structure of stands was based on frequencies of nuclear microsatellite (SSR) alleles occurring in three DNA loci. The total genetic differentiation of Schrenk spruce populations was low (FST=0.0651). Eight main...
Mitochondrial DNA marker was applied to 10 populations of silver fir from Poland and one from Belarusia. These populations were located within and beyond the natural range of silver fir. The marker exhibited two highly conserved alleles (first – 230 bp and second – 150 bp) based on an insertion−deletion of 80 bp in the fourth intron of the mitochondrial nad5 gene. The geographical distribution of...
Badano występowanie genów vanA, vanB, vanD, vanE i vanG metodą PCR u wankomycyno-opornych szczepów Enterococcus faecium izolowanych z materiału klinicznego pochodzącego z wybranych oddziałów Szpitala Dzieciątka Jezus w Warszawie oraz ustalono podobieństwo szczepów metodą analizy polimorfizmu restrykcyjnych fragmentów genomowego DNA za pomocą (RFLP) - PFGE
Opracowanie i wprowadzenie nowocześniejszych metod badawczych, przyczynia się do dokładnego poznania zmienności genetycznej genomów roślinnych. Analiza molekularna na poziomie DNA pozwala ocenić zmienność genetyczną z pominięciem obserwacji fenotypów i wyeliminowaniu wpływu środowiska, które często modyfikuje ekspresję genów. Zastosowanie markerów molekularnych umożliwia bezpośrednie wykrywanie różnic...
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